Phytochemical Profile, Antioxidant and Anti Proliferative Studies in Different Extracts of Artocarpus kemando Miq. Bark

In this study, the stem bark of Artocarpus kemando was used to find alternative antioxidants from natural sources with fewer side effects. A. wasextracted successively using hexane, chloroform and methanol solvents, evaluated for antioxidant, cytotoxic, antiproliferative activities. The extracts were investigated determination their total phenolic content (TPC) flavonoid (TFC). Then, antioxidant activities chemical based assays such as ferric reducing power (FRAP), capacity, radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH) 2,2’-azino-bis (3-ethylbenzothia zoline-6-sulphonic) (ABTS), β-carotene-linoleic acid (BC) assay, oxygen absorbance capacity (ORAC), cell assay. cytotoxic study done four different lines namely human estrogen receptor positive (ER+) breast cancer line (MCF7), ovarian (CAOV-3), promyelocytic leukemia (HL60), normal immortalised surface epithelial (TI074), microculture tetrazolium salt (MTT) before morphological change on CAOV-3 cell. extract displayed most promising activity compared other when tested DPPH, FRAP, ABTS, TAOC, BC,ORAC, cytoprotective assays. remarkable showed by might be due its high compounds at 855.5 ± 0.01 GAE μg/mL 145.45 0.06 QAE μg/mL, respectively. Nevertheless, better IC50 value 618 0.04 in DPPH Each analysed Gas Chromatography Mass Spectrophotometry constituents obtained then analysed. activity, a comparable cytoprotection ascorbic against free radicals lowest effective concentration (EC50) 21.48 μg/mL. However, cytotoxicity only significant toxicity cells 27.9 0.03, 24.1 0.02 9.0 after treatment 24, 48, 72 h, found capable inducing apoptosis shown membrane blebbing, chromatin condensation formation apoptotic bodies. results that could potential antitumor agents particularly cells.